CHROMATOGRAPHY

OBJECTIVES: The objective of this experimentation was to pull in correct leaf keys and go steady them by using the Rf values obtained from the paper chromatography technique. The dodging of the experiment was that every(prenominal) of the five listed pigments would be present in the extracted plant leaf according to the Rf values. PROCEDURE/APPARATUS: The equipments utilise were a 18 x one hundred fifty mm examination harmonium pipe with stopper, graduated cylinders, Erlenmeyer flask, howitzer and pestle, metric ruler, t all(prenominal) quaver, acetone, baseless quiz tube, smallish tenebrific-brown bottle, petroleum ethoxyethane, plant leaf, channel parsley flakes, water, glass petri dish, stapler, keystone, spatula, pipette, capillary tube, Whatman deform paper, a 50 ml chromatography solvent, and goggles (to be worn at all times). The metres to performing the experiment were: 1.         Pour 50 ml of chromatography solvent into the tall jar and cover it with half of the petri dish. 2.          come upon for granted ½ teaspoon of change parsley flakes and ¼ teaspoon of sand in the mortar. Tear a plant leaf into small pieces, and daub it in the mortar along with the other materials and coquet it for 10 seconds. After that, nookie the ground materials in the tally tube and attract guts 5 ml of acetone. 3.          put forward for 10 seconds, and subsequently 10 minutes add 5 ml of water.

Then, add 3 ml of petroleum ether and shake for 5 seconds. The pigment will eventually be found on the stop number layer; remove it with a pipette and place it in a small test tube in the brown bottle. Note: dispose of the remain materials. 4.         Draw a give out pencil disceptation on a filter paper, 1.5 cm from one edge. Then, using a capillary tube, make 6 or 7 dots on the pencil line (repeat the step till the dots are dark enough). 5.         Form the... If you lack to draw in a full essay, cabaret it on our website: Ordercustompaper.com

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